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1.Food-borne microorganisms

1.1 Multiplex bacteria detection using one-pot CRIS-PR/-Cas13a-based droplet microfluidics

1.2Rapid detection of Salmonella with RecombinaseAided Amplification

Multiplex bacteria detection using one-potCRISPR/Cas13a-based droplet microfluidics

Abstract:High-throughput detection of bacteria at low levels is critical in public health,food safety,and first response.Herein,for the first time,we present a platform based on droplet microfluidics coupling with the recombinasealded amplification(RAA)-assisted one-pot clustered regularly interspaced short palindromic repeats togetherwith CRSPR-associated proteins 13a(CRISPR/Cas13a) assay,and droplet encoding strategy for accurate andsensitive deter mination of nudeic adidsfrom various foodborne pathogens.The workflow takes fulladvantageof CRISPR/Cas13a signal amplification and droplet confinement effects,which enhancesthe detection sensitivi-ty and enables end-point quantitation.Meanwhile,by varying the color of droplets,the number of bacteriadetectedatthe same time is greatly improved.Ilt possesses the capability to simultaneously detectseven differ-ent types of foodborne pathogens.Notably,the system is also applied to real food samples with satisfactoryresults.Overall,in view of superiorities inhigh sensitivity,outst anding selectivity,and large-scale multiplexing,the one-pot CRISPR/Cas13a-based droplet microfluidic system could be expanded and universalized for identi-fying other bacteria.

Key words:Bacterla,Multiplex detection,RAA,One-pot CRSPR/Cas13a assay,Droplet microfluidics,Drapletencoding.

Rapid detection of Salmonella withRecombinase Aided Amplification

Abstract:Rapid Salmonella detection using Recombinase Aided Amplification was established.The reactioncompletes in20 min at 39 ℃ and can be performed with a portable device.Once further impraved,this methodshould be a great choice for monitaring contamination,such as foodborne Salmonella or for similar purposes.

Keywords:Salmonella,Re combinase Aided Amplification,Foodborne,Food safety.